Who we are
ALICe is a full-service imaging platform that integrates a series of innovative fluorescence microscopy tools into a single pipeline. The platform images whole organs at high temporal or spatial resolution and allows 3D data exploration and analysis in a virtual reality (VR) environment.
We are a multidisciplinary team including physicists, neuroscientists, biologists and engineers.
What we offer
ALICe offers cutting edge technologies to researchers including:
- Tissue clearing
- Imaging tools for large cleared samples
- Workstation and software for 3D big data exploration and analysis
- VR tools to interactively explore and analyze 3D datasets
Microscopy facility staff can provide all ALICe services – or a subset – as required.
We offer a tissue clarification service based on the CLARITY protocol. Two X-CLARITY tissue clearing systems from Logos Biosystems are available to researchers.
We have recently expanded our service to support users with existing DISCO-based clarification protocols.
Imaging with mesoSPIM
This custom built lightsheet microscope, developed in Fritjof Helmchen’s lab at the University of Zurich (UZH), is optimized for large scale screening of cleared samples at cellular resolution at high temporal resolution (10 min for a whole mouse brain). For more details see the mesoSPIM datasheet.
Find out more at mesoSPIM.org
Imaging with Clarity Optimized Light-sheet Microscope (COLM)
This custom built lightsheet microscope (originally developed by Raju Tomer in Karl Deisseroth’s Lab – see tomerlab.org) is optimized for large scale clarified tissues. It offers neuroscientists a unique opportunity to capture high spatial resolution (diffraction-limited lateral resolution), whole brain fluorescence images without perturbing neuronal architecture. For more details, see the COLM datasheet.
Imaging with LSM-880 clarity module
We offer a specific module for imaging cleared tissue sections or organoids using Zeiss LSM880 confocal microscope.
A custom-built tissue holder combined with a clarity objective and sensitive detectors allow researchers to scan their cleared samples at high spatial resolution in a confocal system.
3D big data exploration
We provide to researcher high end and efficient software solutions for lightsheet data visualization and analysis.
FIJI, Terastitcher, Imaris and Arivis are included in our workstations.
As part of the ALICe platform, we have developed the VISTO (Virtual HIstology) project, a prototype VR-based analysis tool to interactively view and analyze raw fluorescence data in an immersive, interactive, 3D environment.
How we work
ALICe is a pay for service platform – available to anyone from both academia and industry, worldwide.
You can submit your details in the application form.
ALICe scientific coordinator: Stéphane Pagès, PhD
ALICe microscopy facility manager: Laura Batti, PhD
VR facility manager: Gilles Reymond, PhD
A selection of representative images from the Wyss Center’s lightsheet microscopes:
Publications that include data from ALICe:
Journal of Neural Engineering, 2018, Multilayer 3D electrodes for neural implants
Nature, 2018, Required growth facilitators propel axon regeneration across complete spinal cord injury
Nature neuroscience, 2018, Cortico-reticulo-spinal circuit reorganization enables functional recovery after severe spinal cord contusion
Proceedings of the Lightsheet Microscopy Workshop 2018, edited by the ALICE team and published as a Frontiers Research Topic: Lightsheet Microscopy: from technological developments to prospective applications
Preprint BioRxiv The mesoSPIM initiative: open-source light-sheet mesoscopes for imaging in cleared tissue
19 – 20 March 2018, Campus Biotech, Geneva
Wyss Center lightsheet microscopy workshop (First Edition)
24-25 Apr 2019, Irchel Campus, UZH Zürich
Swiss Light-sheet Microscopy Workshop (Second edition)
Where we are
We are based at Campus Biotech, Geneva.