ALICe

Advanced Lightsheet Imaging Center

Full service lightsheet imaging platform, from sample clarification to 3D VR visualization.

ALICe images

Who we are

ALICe is a full-service imaging platform that integrates a series of innovative fluorescence microscopy tools into a single pipeline. The platform images whole organs at high temporal or spatial resolution and allows 3D data exploration and analysis in a virtual reality (VR) environment.

We are a multidisciplinary team including physicists, neuroscientists, biologists and engineers.

The center is based at Campus Biotech and is under the scientific supervision of Professor Anthony Holtmaat (UNIGE), Professor Christian Lüscher (UNIGE) and Professor Grégoire Courtine (EPFL).

Credit: Newton Cho – Courtine Lab (EPFL) and microscopy staff.

What we offer

ALICe offers cutting edge technologies to researchers including:

  • Tissue clearing
  • Imaging tools for large cleared samples
  • Workstation and software for 3D big data exploration and analysis
  • VR tools to interactively explore and analyze 3D datasets

Microscopy facility staff can provide all ALICe services – or a subset – as required.

Tissue Clearing

We offer a tissue clarification service based on the CLARITY protocol. Two X-CLARITY tissue clearing systems from Logos Biosystems are available to researchers.

We have recently expanded our service to support users with existing DISCO-based clarification protocols.

Imaging with mesoSPIM

This custom built lightsheet microscope, developed in Fritjof Helmchen’s lab at the University of Zurich (UZH), is optimized for large scale screening of cleared samples at cellular resolution at high temporal resolution (10 min for a whole mouse brain). View mesoSPIM details here.

mesoSPIM.org at Campus Biotech developed by Fabian Voigt in Fritjof Helmchen’s Lab (UZH).

Imaging with Clarity Optimized Light-sheet Microscope (COLM)

This custom built lightsheet microscope (originally developed by Raju Tomer in Karl Deisseroth’s Lab – see tomerlab.org) is optimized for large scale clarified tissues. It offers neuroscientists a unique opportunity to capture high spatial resolution (diffraction-limited lateral resolution), whole brain fluorescence images without perturbing neuronal architecture. View COLM details here.

Clarity-Optimized Lightsheet Microscope at Campus Biotech (modified from original Raju Tomer’s design).

Imaging with LSM-880 clarity module

We offer a specific module for imaging cleared tissue sections or organoids using Zeiss LSM880 confocal microscope.

A custom-built tissue holder combined with a clarity objective and sensitive detectors allow researchers to scan their cleared samples at high spatial resolution in a confocal system.

3D big data exploration

We provide to researcher high end and efficient software solutions for lightsheet data visualization and analysis.

FIJI, Terastitcher, Imaris and Arivis are included in our workstations.

VR tools

As part of the ALICe platform, we have developed the VISTO (Virtual HIstology) project, a prototype VR-based analysis tool to interactively view and analyze raw fluorescence data in an immersive, interactive, 3D environment.

The VISTO VR integrated volumetric visualization and analysis tool developed by Gilles Reymond, PhD, Wyss Center.

How we work

ALICe is a pay for service platform – available to anyone from both academia and industry, worldwide.

You can find the application form here.

Main contacts:

ALICe scientific coordinator: Stéphane Pagès, PhD

ALICe microscopy facility manager: Laura Batti, PhD

VR facility manager: Gilles Reymond, PhD

If you would like to find out more, please contact us: microscopy@wysscenter.ch

ALICe images

A selection of representative images from the Wyss Center’s lightsheet microscopes:

ALICe images

Publications

Publications that include data from ALICe:

Journal of Neural Engineering, 2018, Multilayer 3D electrodes for neural implants
Nature, 2018, Required growth facilitators propel axon regeneration across complete spinal cord injury
Nature neuroscience, 2018, Cortico-reticulo-spinal circuit reorganization enables functional recovery after severe spinal cord contusion

Where we are

We are based at Campus Biotech, Geneva.

ALICe is embedded in the Wyss Center’s Microscopy Facility, part of the Preclinical Neuroscience Platform (PNP), at Campus Biotech.

brain imaging
Credit: Microscopy Staff and Holtmaat Lab, UNIGE

Get in touch

We welcome informal enquiries.

Contact Stéphane Pagès or Laura Batti to find out more.

Contact us